How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to use multiple tables to generate a graph in Graphpad Prism?

I have two different tables: one for metabolic activity of the cells in a plate, the other one is the Total DNA assay from the same plate. I need to generate a graph which represents metabolic...

29 January 2021 3,294 5 View

How could i explain the EC50 data utilized by graph Pad prism 9 ?

I measured the FARP antioxidant activity of plant extract than i used graph pad prism 9 to calculate the EC50, now i am not sure how to describe these data as i got the logECF and ECF .

17 January 2021 4,576 3 View

Basic Statistics - Test Selection for the comparison of two Groups?

I could not find a question related to my problem so I am asking in a new thread. If the question has been asked before please direct me to the concering thread. I have no experience in...

02 December 2020 1,567 5 View

How to create a metal binding curve for a metalloprotein using prism?

hello, I need to create a binding curve to measure the affinity of my enzyme to its zinc cofactor, to determine the Kd value. I'm using prism (graphpad) and there are a lot of options under the...

14 November 2020 2,120 3 View

Need help plotting long lists of data in Graphpad Prism -?

Hi, I am generating long lists of per-cell data that I'd like to visualize as dot plots per condition. The problem is that my conditions are defined by letters rather than numbers. Or sometimes I...

11 November 2020 6,707 1 View

Can I display regression line for a whole group, when subgroups are displayed on Graphpad Prism?

I have created a scatter graph in Prism under -> multivariable analyses -> extract and re-arrange, to display relationship between 2 variables, the 3 subgroups within the group are...

16 October 2020 310 1 View

Software freely available for the Statistical analysis?

Can anyone tell me which software I use for statistical analysis except the graph pad? GraphPad software is having 30 days trial.

11 October 2020 740 4 View

Do I have to assume sphericity or not when conducting two way repeated measures ANOVA in graphpad prism 8?

I am analyzing data obtained from a crossover study conducted on same animals evaluating the effect of two different anesthetic drugs on heart rate, respiratory rate, pulse oximetry, rectal...

28 September 2020 6,408 4 View

Help to produce graph in ORIGIN PRO?

Hi, I want to produce a graph in Origin, comparing two groups of data, in the form of scatter points, where the mean and SE bars are included. See attached photo for reference. It is simple to...

25 September 2020 9,756 1 View