23 January 2019 1 267 Report

I have whole genome bisulfite sequencing (WGBS) data for a species without reference genome. Is there any pipeline that I can use to process my data ?

More Laxman Adhikari's questions See All
I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,251 1 View

How to prevent co-precipitation of petide drugs spiked in plasma (or PK samples) during extraction process?

These days, I am working on peptides. While preparing the standard, I have been facing no detection of peptides in UPLCMSMS analysis. Actual case: Case 1: Water + peptide STD working solution +...

04 June 2024 6,453 0 View

How to estimation of CO2 fluxe and H2O fluxes in crop grown areas and its calculation?

Quantative estimation of energy balance, water balance and co2 fluxes during various stages of crop

12 March 2024 7,490 0 View

What does Habitat Occupancy mean? How to calculate Habitat Occupancy for a small animal species?

I want to assess the habitat occupancy of a small species in a habitat. What should I collect as field data to assess its habitat occupancy?

06 February 2024 9,039 4 View

Why has development cooperation been less productive in less developed countries?

Is it because of the weak governance of the recipient countries or the hidden interests of the development partners that influence the policies?

28 December 2023 378 3 View

How can I access it to publish my article?

I want to publish my academic article in this journal. How is it possible?

26 December 2023 421 4 View

What is the specific impact of microsatellite marker analysis on unraveling the genetic diversity and population structure of sheep in Nepal?

What is the specific impact of microsatellite marker analysis on unraveling the genetic diversity and population structure of sheep in Nepal, and how does this information contribute to the...

26 November 2023 3,797 0 View

Likert scale data analysis and multi-level linear regression adequacy for this data type?

Dear all, I have the following data and questions related to the issues in the results. Independent variable I did an online survey and asked parents questions about neighbourhood-built...

26 September 2023 4,919 4 View

Why Big Data are to be secured? Is it any threat to National Security?

Data Security Big Data

14 August 2023 2,922 6 View

How to implement map reduce algorithm on hypercube network in matlab/ python?

Map reduce framework need to be implemented on hypercube network architecture in MATLAB or Python progeamming

14 July 2023 8,137 5 View

Similar questions and discussions
Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close