Hello
Just wondering when I stimulate the HL60 cells with DMSO and use them around D7, do you advise to change the medium just like how we normally pass down the cells? Or should I keep them in the same medium until I use them?
And whenever I change medium is it necessary to add DMSO?
Because I found that most of my cells were dead if I don't change the medium over time
Can somebody advise me on this?
Thank you
A similar question was answered 4 years ago. Keep them at 5*10E5 per ml in DMSO containing medium (See E.Eskati). I suggest you monitor their differentation status and viability each day. 7 days may well be too long, you don't want to be using them for population assays if there are more than 10% dead cells.
A protocol for HL60 cell culture and differentiation (with 0.8% DMF) has been described in detail by the university of Alabama at the following link https://www.vaccine.uab.edu/uploads/mdocs/UAB-MOPA.pdf. We get high number of cells, and cell viability close to 90%, with this protocol.
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