My lysis Buffer contains 5M Nacl, 0.5M EDTA, 1M tris ph 10, 10 MNaOH, Then 1% triton and 10% DMSO is added on the day of the experiment.
Electrophoresis Buffer contains 0.5M EDTA, 10M NaOH and 1%DMSO
Samrat Das
Dear Mie Monir,
As per the description of your specific problem, it seems that most likely your sample cells are not getting lysed properly. During the lysis step, Please ensure that you keep your sample slides in the lysing solution for a minimum of 2 hours (at 4 degrees Celsius).
Best Wishes,
Samrat
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