Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

DoE: Possible reasons for having a lot of significant effects? Is it as complex or went something wrong?

I did a product development using Design Expert for planning and evaluation. There is a response with a lot of significant effects shown in the Half Normal Plot. By testing factors A, B, C, D I...

06 December 2020 5,128 3 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

What do i do when Gaussian 16 shows error during frequency calculation for lanthanides using mixed basis set?

I am trying to do frequency calculation after optimization calculations using mixed basis set for lanthanides SDD and 6-311++G(d,p) for other atoms with %mem=2GB. The error shows: Not enough...

21 October 2020 9,578 3 View

Controlling for a variable that is an outcome of the dependent variable: what is the interpretation of the coefficient of the regressor?

I am estimating a dependent variable as a function of a regressor using Ordinary Least Squares regression. If I add a control variable that is (partly) an outcome of my dependent variable, how do...

06 October 2020 3,224 1 View

How to interpret Nyquist plots given by BODE 100 for charged and discharged cells ?

I have two Li-ion cells one charged and other discharged, same chemistry anodes and same manufacturer. I have Nyuist plots ontained by single sweeps in Bode 100 and also Frequency plots. How do I...

06 September 2020 1,122 1 View

Which EEG source analysis plugin for Matlab do you recommend?

Good morning, I have the EEG recordings of 65 subjects and I would like to reconstruct the source of the signal in the gamma frequency range. Unfortunately, I don't have an individual MRI or...

25 August 2020 10,021 2 View

Is it possible somehow to maintain Maximum Power Point of solar panel while not using the buck-boost converter?

I am looking for a method or component that helps maintaining MPP while charging battery from solar power without using buck-boost converter (may be a cheaper device can be used) Can anyone give...

01 June 2020 9,922 5 View

Botany - Betula pubescens old drawing?

Does any of you have a vintage botanical print of Betula pubescens (alba) subsp. celtiberica? Thanks a lot!!!

27 February 2020 6,049 5 View

Why do I obtain wavy bands on acrylammide precast gel in western blot experiments?

Hello! I recently tested a new precast gradiented gel for my Western Blot experiments and I obtained wavy bands both for proteins and for molecular weight marker. I run in tris-glycine-sds running...

28 July 2019 2,303 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

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28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View