Hi everyone,
Currently I'm culturing cells in a 3 mg/ml fibrin hydrogel. However, the cells either destroy the gels (without any anti-fibrolytic compounds) or compact the gels when anti-fibrolytic compounds are added (I've tried different concecntrations of aprotinin and 6-aminocaproic acid). Has anyone found ways to address these issues? I've found that I'm unable to just raise the aprotinin/6-aminocaproic acid levels because it ends up being detrimental to cell viabilities.
Moreover, I see many papers culture cells (including fibroblasts) in fibrin gels seemingly without issue! I wonder if these differences could be due to fibrinogen product/source variability :/
Thank you all!
(The attachment is an example of a compacted fibrin gel)
my friend Jörk-Ulrich Wieding observed fibrin-excess in bloodplasma, treated with sun-light. Seemed to be an interaction of physical triggers and chemical bounds.
What does unfractionated heparin change?
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology
- Culture Cells