Hi,
I am designing an experiment for intracellular cytokine staining. My question is, after culturing cells for 24-48 hours, can I use trypsin-EDTA on splenocytes to dettach cells?
Any input will be appreciated.
Thank you.
Nicole Acuff
Hi Lekha,
Yes, you can use EDTA or trypsin to detach cells from a cell culture plate or flask. Most splenocytes, except macrophages and DCs, are loosely adherent so you can also pipette up/down to resuspend these cells.
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Lekha Shrestha
Thank you Nicole. Will try using EDTA. Do you particular concentration that is leas toxic for the cells.
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