Could I use 2ndry Ab (Goat Anti-Rabbit Immunoglobulins/HRP) several times in detection stage of WB once it is diluted in 5% BSA or milk/TTBS ?

Dear friends I am optimizing WB on liver tissue collected from mice and I need an answer for above mentioned question according to your expertise?

07 September 2020 1,607 3 View

New methods for measurement hepatic insulin sensitivity both in vivo and cultured cells?

hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...

21 December 2019 6,557 1 View

What is the best way for extraction RNA from bacterial culture?

Could anyone recommend the best extraction kit for me in order to extract RNA from bacterial culture? Your cooperation is highly appereciated. Thanks in advance.

02 March 2019 3,508 3 View

No title

11 December 2018 6,646 0 View

How can we measure insulin sensitivity in adipocyte culture?

If we manipulate gene expression in adipocyte culture to affect insulin sensitivity. Which are the suitable methods for measuring insulin sensitivity??

08 September 2018 1,056 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View