What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Which factors contribute to instructional resilience in HE?

If you are teaching in Higher Education, please consider participating in our study on Instructional Resilience during Emergency Remote Teaching. We want to learn about which factors have...

19 January 2021 6,759 15 View

How to view the electrostatic potential value of a Gaussian09W result?

Hi all I am able to view the electrostatic potential surface using Gaussview to see the calculating result from Gaussian09W of a small molecule. However, the strength is represented by color. I am...

19 January 2021 5,448 3 View

Statistics Question: Meta-analysis of factorial trials?

Hi all, is it possible to do a meta-analysis of randomized controlled trials with factorial design? I would appreciate some guidance/referral to a statistical resource or guide on this. Many thanks.

17 January 2021 4,090 3 View

How would I go about equilibrating the internal humidity of a closed system at 2 different temperatures?

I have a box which is kept at 4C which is enclosed in a ziploc bag. The dimensions of the box are: 18cm x 13.4cm x 4.9cm. The relative humidity of this internal environment is 30% at this...

07 January 2021 6,734 3 View

Blue native page transfer problem?

Hello, I am writing this hoping someone can help me. I am just starting out with BN-PAGE and I have a transfer problem. It seems each time I transfer (2 membranes at a time) in a BioRad wet...

09 December 2020 1,008 3 View

What is the influence of reduced graphene oxide (rGO) in a p-n ternary photocatalyst?

04 December 2020 9,019 2 View

Can I use both coarse resolution (1 km) and high resolution predictors (2 m) in a species distribution model?

I am proposing that a hierarchical approach is used where coarse resolution variables are used to run a maxent SDM and delineate a presence/absence map for my species (tropical conifer tree...

24 November 2020 1,106 4 View

Are there any interesting areas of research yet to be studied with rice viruses?

I'm currently in the process of coming up with a research pitch for a lab to be funded here in the Philippines. Rice is quite the common crop in the South East and its diseases are commonplace....

23 November 2020 5,194 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View