In our lab, we use Corning 3903 White 96 wells plate to culture cells and use Celltiter-Glo (Promega G7570) to test cell viability, I wonder if we could use normal clear 96 wells plate, such as Corning 3599 to do the same test? I know it's better to use white plate to do the luminescent test, but it is also very burdened, thank you!
Hi Mingui,
I used clear plates a couple of times but I did notice that some values were strange for no actual reason, forming a cloud like increase around high values, though not of substantial difference. Then I changed to black plates with a clear bottom because I wanted to reduce noise as much as possible (but white plates are also fine). So far I haven't seen clouds of higher values, like with the clear plates. But if it makes it easier, try it one time and find out how big the difference is? It may depend on your cell density, type etc.
good luck and enjoy the proces! :)
Dear Minghui,
We tested this thoroughly and i would not recommend using clear plates. The crosstalk from high values to low values has a strong impact if you have a randomized plate layout, since many low and high values are mixed. Attached you can find an example.
If you have to use clear plates it is crucial, that all high values are together seperated from low values and vice versa otherwise you will get crosstalk between wells.
There are also hybrid plates with white wells and clear bottom which work fine if you wanna have a look on your cells.
Best Regards,
Niklas
Any fluorescence (and somewhat bioluminescence) assay will be problematic in a transparent multititter well plate as you will have signal 'leak' from well to well wherever you have an active signal (i.e. fluorescence or bioluminescence). Transparent plates work well only for absorbance, where the signal is passive.
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