Can anyone help me in identifying the type of cells please?

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How to troubleshoot dark background and black patches while performing western blot?

Hello researchers, I'm currently working on a project involving LC3B antibody (Cell Signaling & SIGMA) for western blot analysis. Unfortunately, I've been consistently facing issues with dark...

24 January 2024 929 4 View

What is the best methods for measuring the solubility of ionic solids in organic solvents?

Hi, I am trying to measure the solubility of materials such as Li2S and P2S5 in an organic solvent such as Acetonitrile? What would be the best method for doing so using UV- Vis spectroscopy?...

09 November 2019 8,002 1 View

Has anyone seen no response of HaCaT cells to FCCP in a Seahorse mito stress assay?

Hello, I performed a titration of FCCP in HaCaT cells in order to do a mito stress test with the Seahorse technology (Agilant make). I have tried 0.125, 0.25µM, 0.5µM, 1 and 2µM concentration of...

15 October 2018 7,388 2 View

What is an example of a safe synthesis for a nontoxic(Non polyurethane)?

Safe is referring to the monomer and other components also being relatively harmless and not explosive, or extremely reactive to air or other lab environment conditions. Links to papers and...

16 April 2017 365 7 View

What is the most efficient method to culture HepG2 cells? 10% FCS in DMEM or 10% FBS in DMEM?

Hello, I'm going to culture HepG2 cells soon, I studied many research articles but half of them culture HepG2 in 10% FBS in DMEM and rest of them used 10% FCS in DMEM. I was wondering as HepG2 is...

17 January 2017 6,993 10 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View