I want to knock out a gene using CRISPR/Cas9 in detached chili leaves. I used Agrobacterium to insert the CRISPR/Cas9 system using syringe infiltration and incubate in 28 C for 2 days. The PCR result showed that the Cas9 successfully inserted to the genome but there is no sign of success knock-out. We don't have Cas9 antibody to check for Cas9 expression in western blot. What should I do?