Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

What can be the Research Design if I am looking for Correlation Coefficient and Regression Coefficient?

In our research our title includes the impact of academic anxiety on academic performance. This one is a quantitative research. Our data is numeric. Can someone suggest what can be the research...

21 January 2021 1,650 3 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

Can someone give me an example for a questionnaire regarding roger's innovations in organizations?

Hello, I am looking for a questionnaire based on Roger's Diffusion of Innovations - Innovations in Organizations theory. Can someone give me an example?

15 September 2020 8,351 4 View

Are there studies on fallback fruits of avian frugivores?

Hello everybody! Fallback fruits are fruits of low nutritional value that in periods of fruit scarcity become the filler or staple of a frugivorous animal. Fallbacks seem to be well-studied in...

17 August 2020 7,923 4 View

Can I get some help and reading these results? I ran a regression analysis of students pre and post test grades?

After conducting a linear regression analysis of Interpretive Reading (Pre-test) scores (Table 12), the coefficient or Y-intercept associated with the pre-test scores, (0.696985136) is zero, and...

02 July 2020 7,309 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

What molecules bind to dsDNA?

Hello, there are some molecules that bind to double-stranded DNA; for instance, SYBR green and some antibodies. Are there more examples? Thank you

25 January 2021 7,595 4 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View

Transcription mediated amplification (TMA)?

I am trying to optimize TMA in my lab. When I start with 150 pg of DNA (Size 235 bp), I get good amplification, which I can visualize as an equivalent size dsDNA product, gel pic attached....

18 November 2020 7,622 3 View

CD of insoluble aggregate?

Dear all, I would like to characterize the potential structure of a insoluble aggregate in biorelevant media. I was wondering if I could do this with circular dichroism and how to do it. Do you...

06 November 2020 4,377 3 View