Sir if your question is related to arbuscular mycorrhiza fungus, the in vitro monoxenic culture of AM fungus can be obtained with carrot hairy roots, in which an average of 8500–9000 R. irregularis spores can be obtained from 10–15 parent/mother spores. However, studies suggest that spore production in non-symbiotic culture systems remains lower than that in symbiotic co-cultures.
I have also shared a related research article for you which might help you regarding this