Recently found a processed pseudogene in which exon 1 was not present. Happy to hear possible explanations.
Time for a literature search! See what is already known about your pseudogene of interest (or processed pseudogenes in general).
No shortcuts to success - you'll have to see what is already known.
Good luck!
PVD system, when opening the oil diffusion pump, the measured value of the ion sensor fluctuates greatly at 9.0-1pa and 7.0-1pa, the system does not leak. cooling water 30 degrees. I was very...
04 April 2024 647 2 View
The source material is a ceramic glaze pattern,After cleaning and drying at 500 degrees for 8 hours. They are fed into a TiN PVD furnace, the target is 20 plasma sources! The plating furnace in...
16 June 2023 5,001 5 View
we coated titanium gold by sputtering method, recently the surface is very bad, maybe it's because the surface is not clean? soiled?
23 April 2023 5,563 1 View
Recently, after each coating, the plating cavity appears blue purple, we use Agron and N2 to cover the yellow color.
08 April 2023 6,759 4 View
why when vacuum heating. suction power of vacuum pump is reduced???Why heat the vacuum plating chamber? during heating to 150 degrees the value of the vacuum chamber is at 3.0x10-2 pa , when...
05 March 2023 8,116 2 View
We are coating 24k Gold on ceramics with TiN, the gold color is not beautiful with deep color, Gas ratio is 200sccm(Ar)/500sccm(N2)-(3min)/(5min).Is this ratio necessary. to tweak???Thank!!
24 January 2023 787 1 View
What is the correlation between SULT1A1 copy number and enzyme activity when measuring the copy number using Taqman probes?
24 April 2017 9,760 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...
15 July 2024 182 1 View