1) Acetaldehyde and acrolein both are very evaporable and there are some loss during the solution preparation and incubation with cell in the 96-well plates or other plates. So A549 cell survival rates ( or other test endpoints ) always show different (or inconsistent or ruleless) results under the same test condition in the different repeated tests. Aldehyde solutions were made with the current use! It is very dangerous to operate with aldehyde solution, especially when adding this solution in the 96-well plates or other plates due to their evaporable nature. Do you have the same experience in the similar experiments? And how do you deal with it? Could you give your some good advice or your good experience to me?
2) Maybe as what we have saw, it is obviously to find the culture medium is seriously metamorphic when using the culture medium (1640 medium is used to feed A549 cell by us) to prepare the stock solution of aldehydes. Can the results be true and accepted under this situation? Or may you have some different methods to avoid this situation?
http://www.ncbi.nlm.nih.gov/pubmed/23144150
http://publica.fraunhofer.de/documents/N-150005.html
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