HI everyone,
Could I have your opinion on these photos please ? I revived my HEK293T cells yesterday in FreeStyle medium and I noticed today those black floating vesicles. I had contamination previously but it was completely different, bacteria were forming a "floating carpet", medium was creamy white. But this time it's not like that at all, so I was hoping it will be just some debris cells or maybe stress granules resulting of the thawing process.
Thanks for your help !
Mel
I work wih HEK293, U87 and SVGA and sometimes I get the same after thawed them but it disappear with the passages.
I don't think this is contamination. Try detatching your cells after washing with PBS with Ca2+, and pelleting them at lower rpm. If black spots disappear, it is unlikely it is bacteria.
Dear Melanie,
I don t think it is a contamination. Maybe it is cellular debris floating. Thawing new cells might be the best option in this case.
Thanks everyone for your answers,
Turns out it was a contamination (I've inoculated nutrient blood agar plate and it came out full of colonies). I could clearly see those black bacillus "swim" in the contaminated flask. I'm not using antibiotics so I guess eventually this had to happen.
Thanks again for your help.
Atb.
Mel
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