Hi guys,
I want to measure microtubule bundlings, but I have no clue how to cope with this. Do you know some plugins or methods? Thank you very much for your input!
Cheers,
Feng
Hi Feng,
If you work with fluorescently labelled microtubules in vitro you can measure mean brightness along the microtubules/bundles per unit length. Collect enough statistics and build a histogram. Make a control under similar conditions but with very diluted microtubules and without any bundling agents to measure what brightness corresponds to single microtubule. After determining the brightness of single microtubule you can analyze the brightness histogram from your experiment to see how strong is microtubule bundling.
Hope this helps,
Anatoly
You can always adjust the exposure time of your camera or the illumination brightness to make sure the signals you are measuring are in the linear range.
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