Is it possible to use MOPS buffer or HEPES buffer instead of MES buffer? It's for regeneration of ClonTech TALON Metal Affinity Resin with Co2+?

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Sansrity Sinha

i dont think so..because MOPS and HEPES maintain oh 7.5 approx..whereas MES buffer is used to mainnitn ph of rage 5.5 - 6.7.

Müge Algan

Thank you for the answer ma'am. So, should I use Bis-Tris buffer? Its useful pH range is 5.8-7.2 and it is the closest one to MES buffer.

it shuld work. all the best