Hello everyone, I’m looking for some advice regarding an issue we’re experiencing in our lab with HCT 116 (CCL-247) and FHC (CRL-1831) cell cultures.
We recently onboarded new students, and shortly after, we noticed unusual debris-like structures in our cultures.
In the FHC cells, these structures exhibit locomotion, which suggests possible contamination. Despite washing the cells and changing the media, the issue persists, and the contamination returns within a few days.
We are also culturing HCT 116 cells, where these granular structures are presenting and are adhering to the bottom of the flask.
Has anyone encountered similar issues or have suggestions on how to identify and eliminate the source of contamination? Any insights would be greatly appreciated!
Hi Kendra,
It's look like some Micoplasma contamination in your culture. Micoplasma is common contaminants in cell culture work and need effective screening method to confirm and eliminate it.
Suggested to screen cell seed (mother seed), serum (FBS / NBCS), media and viral seed if you are infecting the culture.
We are using PCR for the detection, which detects 16S and 23S rRNA targets in different mycoplasma species.
You may get some information about micoplasma screening from following research article in my profile :
Standardization of microcarrier based bioreactor culture in parallel with roller bottle for rabies virus (PV-11) propagation in Vero cell using MEM eagle’s and RPMI 1640 medium
Dec 2022
DOI: 10.30574/wjbphs.2022.12.3.0257
Thank you,
We ended up decontaminating and starting with fresh cells and fresh media.
Good to know, that's the only way if the cells contaminated with micoplasma.
Best wishes for your research.
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