When detecting p53 in human samples by Western Blot, the antibody is supposed to show a single band around 53KDa. However, I consistently see extra bands (non-specific signals).
Hi Binbin, p53 can show multiple bands in human samples because it has several isoforms and undergoes various post-translational modifications (such as phosphorylation, ubiquitination, and acetylation). These modifications can shift its apparent molecular weight, leading to extra bands on the blot, even when using a specific antibody. This is a common observation in p53 Western Blots.
The answer to this question comes from MCE Technical Support.