Human sample WB with p53 antibody: Why extra bands besides ~53KDa?

04 August 2025 1 9K Report

When detecting p53 in human samples by Western Blot, the antibody is supposed to show a single band around 53KDa. However, I consistently see extra bands (non-specific signals).

Lucian Miles

Hi Binbin, p53 can show multiple bands in human samples because it has several isoforms and undergoes various post-translational modifications (such as phosphorylation, ubiquitination, and acetylation). These modifications can shift its apparent molecular weight, leading to extra bands on the blot, even when using a specific antibody. This is a common observation in p53 Western Blots.

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