Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Acetic acid in liquid waste ?

Hy, may you suggest a method to quantify acetic acid in wastes or leaching test? Thanks in advance Stefano

10 December 2020 4,023 2 View

Which points I have to take in mind to mutate a plasmid that grow in Neb stable strain?

Several kits that allow the introduction of point mutations in plasmids say to transform mutated plasmids in DH5alpha bacteria. I have a plasmid with many repetitions that need stable strains to...

08 November 2020 4,250 2 View

Did you have any problem for staining nuclear phosphorylated proteins in hippocampal neurones?

I was wondering if there may be a problem of permeabilisation of cells. I am working on mice hippocampus (pups) and I need to make a staining for a phoshoprilated nuclear protein. Since the...

17 September 2020 2,000 3 View

Glass or PVC material to perform a soil leaching experiment of pharmaceuticals?

Hello I am developing a soil column experiment to study pharmaceuticals transport and attenuation.I would like to know If is better to use a glass or a PVC column to perform this experiment and...

10 August 2020 6,214 3 View

How many researchers are interested in attending web communications in the field of Sustainable Planning?

17 June 2020 4,753 74 View

What happens to non-"preclincial essential research" (non Covid19) in Covid times?

Are you askiing what researchers are doing in Covid time especially those that work with animal? I started a survey to collect information (anonymous) to see whether there are alternatives to...

02 April 2020 9,596 3 View

Generic DC/HYBRID grid-connected microgrid available in simulink?

I'm looking for a microgrid simulation model written in simulink in order to implement my simulation data and test my Energy Management System algorithm. The microgrid I am looking forward is a...

24 October 2019 3,541 4 View

Is there an investigation about the real world implementation of decision making algorithms in smart home/microgrids EMS microcontrollers?

Implementation of stochastic and probabilistic based algorithm is a very tricky task especially in energy management systems microcontrollers. I'm looking for a research paper which talks about...

30 July 2019 5,947 1 View

What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

24 June 2019 3,133 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Are there any known solvothermal methods to synthesize MoS2 nanosheets using solvents other than water (like ethanol/ethanol-water mixture) ?

I need to synthesize MoS2 nanosheets by alternate methods without pure water as a solvent.

22 February 2021 9,246 1 View