Is running several one-way ANOVAs justifiable when an (appropriate) two-ways ANOVA does not reach statistical significance?

Hi, We have been discussing this topic on my lab for a long time. Is there any justification for reporting statistically significant results of several one-way ANOVAs when a previous and more...

29 January 2020 8,458 2 View

Choosing compound' doses for rats when only mice-related bibliography is available?

Hi all, We are planning to systemically deliver a synthetic pharmacological compound in rats. When looking for specific dosage and administration protocols, we only found studies with mice....

05 May 2019 3,644 5 View

TCID50 for Non-forming and Non-CPE virus?

Is there a possibility to calculate TCID50 for non-plaque forming virus, the virus I'm trying to titer is also known to be non-cytopathic, so my questions is: Is there a method of titers this sort...

15 April 2019 8,266 2 View

No title

10 March 2019 7,613 4 View

With SOLID185 microplane damage model. How do I solve my error in Ansys??

In the last weeks I have worked the damage model of the element SOLID65, but it has not allowed any results, so I decided to work with the element SOLID185, with the recommendations of the...

07 March 2019 5,662 5 View

Can someone help me with Immunofluorescence Assay for CSFV quantification?

I'm testing a protocol for viral quantification by IF; it is being standarizing from the fixation step, as a fixative choosen are Methanol (10mins) and 4% Formaldehyde-10%Methanol (15mins) (Is...

26 February 2019 3,020 1 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

27 January 2019 7,520 11 View

I need to extract dna from environmental biofilms from contaminated sites that solution recommend me to clean my samples?

biology

03 April 2017 9,126 0 View

Somebody can recommend me some Books?

I'll become a student (MSc by research in organic chemistry). I would like to know some useful books expecially for the "work in the laboratory" like spectroscopy, how to purify some reagents...

02 March 2017 2,429 0 View

What is the best way to recover low amounts of DNA from an agarose gel band?

10 November 2015 9,428 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View