I have used expired LS columns (2 years after expiration date) and the results have not been so good. I think that the reasons could be my low number of cells and my tissue, but the expired columns could be related?
Thanks
I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...
14 July 2024 3,570 5 View
I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...
23 June 2024 9,584 1 View
I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...
27 May 2024 8,025 1 View
Can Sensory marketing affects customers satisfaction in Hotels?
12 May 2024 3,308 3 View
Hello everyone, I would greatly appreciate it if someone could assist me in understanding the steps required to estimate the sample size for a longitudinal study. Or advise bibliography. I am...
04 April 2024 2,882 0 View
I am using mouse serum to block Fc receptors before staining, for flow cytometry. My question is if I should also add the serum to compensation beads before staining them, so that the cells and...
07 March 2024 9,989 2 View
Most staining protocols for flow cytometry in 96-well plates use V-shape or U-shape plates. I would like to ask if staining could also be done in flat-bottom plates. Thank you!
07 January 2024 2,910 5 View
I plan to run my experimental samples in the flow cytometer in batches. My question is if FMO controls have to be prepared and run every time I run experimental samples, or if I could only do them...
04 January 2024 7,842 0 View
I am immunophenotyping PBMCs by flow cytometry using markers for T cells to analyse the different subsets (Naive, CM, EM and EMRA), and to measure senescent and exhausted t cells. I want to...
02 January 2024 5,387 1 View
Hi, we've been unsuccessful in creating single clones with deletion of a couple of proteins, after testing several sgRNA sequences for each. We are using a lentiviral vector encoding puromycin for...
25 December 2023 2,901 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I want to know more about diamond ore deposits in world.
08 August 2024 1,514 0 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
to identify themes in question with APA style references
07 August 2024 2,239 5 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View