I used a 2 M trifluoroacetic acid solution and hydrolyzed the EPS at 100°C for 2 h. How can I distinguish this method hydrolyzed the samples completely? I don't have access to NMR
You are trying to hydrolyze to component monosaccharides?
check out TLC or paper chromatographic methods. These are rapid and work on small amounts of material.
for carbohydrates you may need to buy reverse phase TLC plates - not cheap but a lot less than an NMR!
Many Thanks dear James E Hanson , my purpose is getting to monosaccharides.
but if we have remaining oligosaccharides in hydrolyzed solution, they won't be distinguishable. because we have standard monosaccharides as markers.
is HPLC applicable here?
Yes it could be. What you probably want is SEC or GPC - the chemical nature of the mono-, oligo and polysaccharides isn’t great so you want to distinguish them by size. The system can separate mono from di from fro but cant tell different monosaccharides apart.
unless someone else at your institution has already done it, you are probably going to have to order a column. Find one that works for saccharides in the desired MW range.
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