Is it possible for IC50 values (for the same data) to be different when calculated by graphpad prism from calculation by Excel sheet (drawing a curve ) or do I have an error in calculating these values.
Hello Mustafa A. Hadid
Yes, there is usually a difference in parameter estimation.
However, the GraphPad software result is more accurate and acceptable.
Of course, you should pay attention to the important point that you have used the appropriate nonlinear regression model in the software.
Abolfazl Ghoodjani Thank you very much
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