How to prove the association between a gene and Chromatin accessibility?

16 December 2021 1 7K Report

I want to use TUNEL staining to prove the association between a gene and Chromatin accessibility. However, I cannot control the experiment volume. What are the other ways to prove the association between a gene and Chromatin accessibility?

Hua Xiao

You can use DNase I hypersensitivity as a measure of chromatin assessibility. Or you can perform ATAC-seq to assess genome-wide profile of open chromatin. These are well-established methodologies. Hope this would help.

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