We want to study the role of the lignin metabolic pathway in drought stress tolerance. Can anyone share their experience about how to determine the role of the lignin metabolic pathway in crops under drought stress?
Dear Adnan,
Lignin metabolism regulated by shikimic acid pathway which involve multiple lignin related enzymes (PAL, 4Cl, POD, and CAD) these four are main enzymes regulate lignin. Our research group study about lignin metabolism under shade stress. You can check out this recently published paper. Hope this would be helpful.
Previous studies have found that increased hydrogen peroxide in the plants after soybean aging or drought stress induced an increase in POD enzyme activity, which in turn accelerates the lignification. (https://www.mdpi.com/2073-4395/10/8/1177/htm#)
Lignin is an important organic polymer which is abundant in cell walls of some specific cells. It has many biological functions such as water transport, mechanical support and resistance to various stresses. Most of the current researches on lignin in plants are focused on the regulation of lignin content through molecular biology and molecular genetics. Reducing the accumulation of lignin in energy plants can improve the production efficiency of biofuels. However, as shown above, the reduction of lignin biosynthesis can seriously affect plant growth and development, increase the risk of crop lodging, reduce plant resistance to external biotic and abiotic stresses and thus result in a serious threat to crop production. The general biosynthesis pathway of lignin in higher plants. PAL, phenylalanine ammonia-lyase; TAL, tyrosine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate: CoA ligase; CCR, cinnamoyl-CoA reductase; HCT, hydroxycinnamoyl-CoA shikimate/Quinatehydroxycinnamoyltransferase; C3H, p-coumarate 3-hydroxylase; CCoAOMT, caffeoyl-CoA O-methyltransferase; F5H, ferulate 5-hydroxylase; CSE, caffeoyl shikimate esterase; COMT, caffeic acid O-methyltransferase; CAD, cinnamyl alcohol dehydrogenase; LAC, laccase; POD, peroxidase.
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