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Questions related to Cell Biology
Hey all, I'm trying to select fibroblasts after transduction with shRNA to integrin a5 but I don't know why, my cells are not dying after treatment with puromycin. I treated even cells not...
06 June 2017 9,816 4 View
Immunofluorescence assay, my target protein locates both in ER and Golgi, I want to calculate the percentage in the two organelle.
06 June 2017 4,069 2 View
growth factors like BMP, TGF and igf etc
06 June 2017 8,388 2 View
i intend to apply mechanical pressure on cell to look at mechanotransduction pathways. i will be using a SILAC based phosphoproteomics. Can the data also indicate that the cell is undergoing...
06 June 2017 9,061 0 View
Differentiation protocol followed is standard: after 2 days of post confluence, differentiation is induced using dexa,Ibmx and insulin combination followed by addition of maintenance media every 2...
06 June 2017 5,347 5 View
I used MDa-MB-231 cells, interested in protein that I want to overexpress by using Adenovirus I want to synchronize the cells to do cell cycle analysis Any Suggested steps ? Thanx
06 June 2017 9,967 1 View
Hi to all, Recently I have read some paper related to extracellular vesicles. In the article, I found that some Transcriptional factors(TF) and other macromolecules can be transported by EV. Given...
06 June 2017 9,384 1 View
Is it possible to slow the proliferation of my cells (adult lung fibroblasts) with low serum media (1% FBS)?
06 June 2017 3,217 4 View
Hello! I need to perform a colony formation assay and to identify as viable cells, I will have to color them with MTT. Do I have to just find the MTT on the colonies?
06 June 2017 200 2 View
We have been able to get the Calcium loading dye within the organoids. However, I have been unable to detect calcium changes with previously published positive control of ATP or ionophores which...
06 June 2017 8,719 0 View
MYH9(NM-IIA) is known for regulating cell migration. It can express both on cytoplasmic and membrane. If I can down-regulate MYH9 expression on cell membrane, will it effect its function?
06 June 2017 2,461 2 View
Hi everyone, I'm looking for anti YAP/TAZ and anti phosphoFAK that would give a good signal in immunofluorescence in confluent MDCK monolayers. Does someone know a good reference? Thanks!Pablo
06 June 2017 5,592 0 View
It is described in the literature that Y27632 proliferates cells and inhibits differentiation. Cell adhesion is also good. But when we experimented, there was no clear difference. I was...
06 June 2017 1,576 3 View
Looking for different methods to reduce the metabolic activity of cells. Primarily HepG2 cells, but muscle or adipose tissue cells either.
06 June 2017 1,648 4 View
I'd like to image ROS accumulation in cells with a fluo dye and I'm wondering if anyone have ever performed cell lysis to mesure ATP after ROS imaging ? Does the ATP quantitation is still reliable ?
06 June 2017 692 2 View
The differentiated bulky 3T3L1 cells is very sensitive. So it seems little bit tricky to get a pellet of these cells.
06 June 2017 9,460 1 View
I treat my cells with chemotherapeutic drugs and I want to control the chemotherapeutic stimulation on my cells. so I'm looking for a test and I heard that we can use doxorubicne on flow cytometry...
06 June 2017 7,095 3 View
I have been trying to isolate human adipose microvascular endothelial cells from human omental and subcutaneous fat. I use CD34 and CD31 immunoseperation techniques for the cells to isolate out...
06 June 2017 6,974 6 View
I want to synchronize my culture of H4-cells without using chemicals and decided to use the mitotic shake-off.I followed this protocol for the mitotic shake-off:...
06 June 2017 7,726 1 View
I wish to find whether someone is having a positive history of using Pen/Strep (at 1%) for in vitro culture of Plasmodium. we have been using Gentamycine, which looks safe for the parasites but...
06 June 2017 2,830 3 View
Studying cell migration of glioblastoma primary cell lines and we're looking for alternatives to FBS to promote adhesion without altering the migration phenotype. Does anyone know the particular...
26 May 2017 6,335 2 View
I am trying to express protein on COS-7 cell membrane with a GFP tag. Can any one help me out on how can I purify the membrane protein for western analysis and may be even for flow cytometric...
23 May 2017 4,594 2 View
Can anyone suggest a method enabling a deprivation of leukocytes from the mouse bone marrow-derived cultures- my cultures contain both populations- some leukocytes and mesenchymal stromal cells, I...
18 May 2017 9,606 4 View
Is there any possible cell surface marker that can indicate that the cell expresses P16? I mean without permeability with ab and damaging the cell? I need the cells later for RNA - seq, so...
18 May 2017 5,696 2 View