Protein Synthesis technique?

What method can you use to measure protein synthesis(translation) of specific protein in cell culture as an alternative to radio-labeled Methionine? Thank you

31 December 2018 3,911 3 View

What is the most dependable (reliable) factor to determine the tumour microenviroment difference between different types of cancer?

The tumour microenviroment is very complex and composed from many bio-factors such as (EGFR, VEGF, chemokines, leuckocytes infilitrations...etc). I did many investigations on couple of tumour...

11 December 2018 452 0 View

Can the micro-motion in dental implant affect the occlussion, and unnoticed bone dehiscence?

Despite the limited motion, but I think in cases of crowded teeth or lateral interferences, these may result in future occlussion problems.

10 November 2018 7,331 5 View

Bromophenol blue for 6X loading protein buffer?

Hello, In order to prepare the Bromophenol blue for 6X loading buffer for protein. Do you use ethanol to dissolve Bromophenol blue or PBS buffer. I made with ethanol but my gel bands started to...

01 February 2018 217 0 View

Cell Viability Assay Standardization?

Hi, How do you normalize your cell numbers in viability assay to the zero hour reading if we start with different number of cells, because after seeding cells in 96 hr, there are different cell...

08 September 2017 7,784 3 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

16 July 2017 1,907 1 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

06 July 2017 9,149 1 View

What is the acceptable Passage number of MDA-MB-231cells?

Hi, I'm wondering what passage number is acceptable for MDA-MB-231 cells, Some labs kill these cells when they are 20-25 passages. Others Suggest it should be below 60 passages Thanks

06 July 2017 8,514 4 View

What is the acceptable Passage number of MDA-MB-231cells?

Hi, I'm wondering what passage number is acceptable for MDA-MB-231 cells, Some labs kill these cells when they are 20-25 passages. Others Suggest it should be below 60 passages Thanks

04 July 2017 8,112 3 View

Hi I want to synchronize my MDA-Mb-231 cells and infect them with Adenovirus with protein of interest any suggested protocol?

12 June 2017 9,422 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View