Is Pen/Strep (at 1%) really appropriate for in vitro culture of Plasmodium?

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How to interpret the results on the secondary structure of ITS2?

We have obtained secondary ITS2 structures for free-living marine nematodes of the genus Oncholaimus. How can we check if the structures are correct? And how can we interpret the result if the...

26 March 2024 2,753 0 View

Does anyone know which building or structure was first in the entire history which was build with concrete filled steel tube columns?

Hi my name is Denis and I hope all of you are doing well I am an honours degree student at Heriot-Watt University Right now I am doing research in Concrete-filled steel tube columns and I am...

19 February 2024 6,753 0 View

Can I upload articles if I'm not the author?

Greetings! So I am one of the editors of a journal and I would like to somehow upload all the articles from every volume to ResearchGate. However, many of the authors of those articles don't have...

31 January 2024 4,518 1 View

How to change the list of scientific interests?

When registering, I did not cancel confirmation of notifications about publications by other employees of my university. As a result, the news feed is filled with the work of my colleagues whose...

21 January 2024 9,385 2 View

David Todd, Experimental organic chemistry, Prentice-Hall, Englewood Cliffs, NJ, 1979. 347 pp. ?

Good afternoon. The book is very necessary for work. Please help me find it in electronic form. David Todd, Experimental organic chemistry. Prentice-Hall, Englewood Cliffs, NJ, 1979. 347 pp.

05 January 2024 3,582 0 View

How to set model parameters from Model Finder for MrBayes script?

The output file for the selected evolutionary models by ModelFinder is extremely confusing. This is what I got: PartitionModelPlen: 58s+tefcdn1_1+tefcdn1_2+tefcdn3_3+rpb1cdn1_1!ModelDNA!rates:...

27 December 2023 5,285 1 View

How does one install JupyterLab on a MacBook Air?

Greetings, For one of my cognitive sciences lab session, I have to install JupyterLab on my computer (a MacBook Air). However I really am lost with how to do it, especially since I'm not tech...

14 August 2023 4,135 2 View

How can I have a mentor in Accounting/Finance research?

I am a lecturer in accounting and finance, but I am weak in research. I wish to have a mentor/collaborator to help me come up

14 June 2023 116 5 View

What is the most appropriate statistical test for measuring the interaction of factors?

Hello, I'm a master student working with fungi. And one of my studies includes the evaluation of the mycelium growth efficiency and biomass production of mushroom strains on different culture...

07 May 2023 1,304 14 View

How to normalize a supernatant(media) in western blot?

Hi, I want to quantify the amount of collagen(s) that got secreted outside of cells to ECM through Western Blotting the media, however I'm not sure which housekeeping gene I can use in order to...

27 April 2023 2,071 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Noaman Naji Aaiz

pen / strep. 1% was used by many studies related with T. gondii suspension for experimental inoculation.

Haifa Juma

this link can help

https://malariajournal.biomedcentral.com/articles/10.1186/s12936-015-0815-z

Younes Beygi Khosrowshahi

pen strep is sufficient for different cultures. The sended link by Haifa is very good.