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Questions related to Cell Biology
I want to treat adipocytes with an inhibitor, and collect media after some time to check if the fat/lipids from the adipocytes are leaked into the media. SO i am looking for a protocol which can...
05 May 2017 6,571 2 View
I am preparing a protocol for microsome preparation from HEK (human epidermal keratinocytes) and would like to know if anybody have experience in this kind of procedure. Like CYP concentration is...
05 May 2017 4,998 2 View
I am trying to grow up monoclonal HEK cells by sorting single cells into 96-well or 384-well plate. However, in the majority of wells the cells simply did not proliferate. In 96-well plate, I...
05 May 2017 8,534 2 View
I'm growing keratinocytes and they aren't sticking to the bottle, they've been suspended for three weeks. I use DMEM with 10% FBS.
05 May 2017 5,986 5 View
I'm culturing primary mouse lacrimal gland epithelial cells in DMEM-F12 supplemented with 10% FBS, 0,4 ug/ml hydrocortisone, 0.1 nm CT, sodium bicarbonate, adenina powder, tripsin, transferin,...
05 May 2017 8,782 2 View
I basically want to know what do T cells of different locations in the body secrete or even in different contexts
05 May 2017 2,509 2 View
We are trying to assay bacteria growing at 37 C in plate reader over time and are having condensation problems. Any ideas?
26 April 2017 1,313 5 View
Hello, I am using now suspended cells called Jurkat T cells and I need to fix them with PFA. The problem that I found is that when I centrifugate after the incubation with PFA I lost most of the...
20 April 2017 1,939 4 View
I'm looking for a stain that can be used in 96 well plates (i.e high throughput) for quantitative assesment of tau tangles in iPSC derived coritical neurons. I've considered Thioflavin S/T but my...
16 April 2017 10,128 1 View
Negative Gel-electrophoresis result
14 April 2017 7,678 3 View
I have several flasks of HeLa cells that have recently begun taking on an altered morphology. I do not know whether it is due to some type of contamination or the result of a mutation of some...
12 April 2017 6,466 3 View
As stated in the question, I would like to know what kind of culturing medium is generally used for culturing primary human VSMC. Currently for murine cells I'm using DMEM with 10% FBS, Pen/Strep,...
11 April 2017 8,037 4 View
Dear researches I see white spots with the naked eye in the culture flask of CHO cells. in the microscope, the white spots are large aggregates of adherent rounded cells. i also notice that the...
07 April 2017 2,558 6 View
It is my first time working with L929. I cultured the L929 from stock purchased from ATCC (CCL-1). I observed these large cells amongst the L929 from P0 up to P2. Not sure what it is but when 80%...
07 April 2017 9,056 1 View
Hi, what marker do you suggest to label recycling endosomes Rab11, Rab11a or Rab4? thanks a lot
05 April 2017 6,299 5 View
How are proteins on the surface of cells able to be studied by TIRF microscopy, without becoming "stuck" to the microscope slide surface?
05 April 2017 9,561 3 View
I transduced the SVF preadipocytes with lentivirus and about a month after transduction and selection, now the survived cells are like this. For one like this I observed small lipid droplets at...
05 April 2017 7,479 2 View
I am culturing mouse fibroblast cell in 10% FBS medium with 1% PSG and 0.1% Amp B. What is the condition for this cell to freeze in liquid nitrogen?
04 April 2017 6,573 5 View
04 April 2017 5,347 2 View
I want to do Fluorescence Activated Cell Sorting on plant roots (based on GFP fluorescence) to collect material for RNA-seq. I would like to select out specific cell type (defined by the...
04 April 2017 1,519 3 View
04 April 2017 8,853 1 View
04 April 2017 3,094 7 View
Hi all, I am trying to get the best dilution which gives me 80% confluency next day for transfecting HeLa cells. I supposed to get cell count 1.7X times the next day (which means if I am seeding...
04 April 2017 8,123 4 View
04 April 2017 3,405 4 View