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Questions related to Cell Biology
Hi all, I was wondering if there are any limitations of growing cells or proliferation in collagen gels placed in 96 well plates. Like cell concentration, etc... Thanks in advance,
06 July 2017 2,690 3 View
Hi all! I'm looking to decrease bacterial uptake by PMNs and I've been using Cytochalasin D with limited success. The bacteria are opsonized, so as far as I know this is driving a CR3-mediated...
03 July 2017 4,323 2 View
In need of a reliable housekeeping gene to normalize my pcr data to. Targeting Gfap and GLT-1 genes.
03 July 2017 693 4 View
I treat my cells with chemotherapeutic drugs and I want to control the chemotherapeutic stimulation on my cells. so I'm looking for a test and I heard that we can use doxorubicne on flow cytometry...
26 June 2017 977 3 View
I have been trying to isolate human adipose microvascular endothelial cells from human omental and subcutaneous fat. I use CD34 and CD31 immunoseperation techniques for the cells to isolate out...
24 June 2017 1,435 5 View
what kind of media and how to culture it?
15 June 2017 8,884 3 View
Hey all, I'm trying to select fibroblasts after transduction with shRNA to integrin a5 but I don't know why, my cells are not dying after treatment with puromycin. I treated even cells not...
15 June 2017 476 5 View
Is it possible to slow the proliferation of my cells (adult lung fibroblasts) with low serum media (1% FBS)?
13 June 2017 300 3 View
the protein expresses specifically in brain and lung.
06 June 2017 9,945 5 View
I wish to find whether someone is having a positive history of using Pen/Strep (at 1%) for in vitro culture of Plasmodium. we have been using Gentamycine, which looks safe for the parasites but...
06 June 2017 9,038 3 View
I'm currently thinking about using flow cytometry to characterize and isolate individual cells from my sample that's encased in a hydrogel. This way, I can examine microbial growth rates among...
06 June 2017 5,712 1 View
Any advice is appreciated. Thanks!
06 June 2017 1,557 1 View
I am working on the cell culture system of A.testudineus. I have to adjust the osmolarity of PBS and medium according to the Serum of the fish. Please suggest me the right procedure.
06 June 2017 4,915 3 View
06 June 2017 4,435 4 View
CELL CULTURE
06 June 2017 9,902 0 View
I ask for instructions on how to impregnate or fix corneal endothelium with anti-PH3 and KI 67 antibodies for study of the corneal endothelial mitosis in different animal species
06 June 2017 5,612 2 View
In human cancer cells
06 June 2017 8,189 4 View
I'm trying to compare cell diameters and volumes of different cell types using an automated cell counter Scepter (Millipore). The counter draws histograms of diameters and volumes on the screen...
06 June 2017 5,633 6 View
Which protocol i can use to isolate protein from mitochondria (COX IV and cyto c?
06 June 2017 2,660 3 View
Hello, I normally usually crystal violet to measure cell viability, but the cell lines have all been adherent. I need to measure viability with suspension lines post siRNA transfection. I was...
06 June 2017 7,539 2 View
Cell Biology Researchers
06 June 2017 958 0 View
Dear all, Does anyone know where can I purchase the RPMI 1640 without glucose and phenol red? So far I only found the RPMI 1640 without glucose but with phenol red. Thank you.
06 June 2017 9,251 2 View
Labile cells are capable of proliferation and do not live throughout life (they have a fixed life span) Stable cells are capable of proliferation on demand and live throughout life of an...
06 June 2017 3,851 5 View
I have cultured endothelial and smooth muscle cells from the aorta of rabbits. There has been an issue with fibroblasts in my cultures out growing cells. Is there and easy or cheap ways to fix...
06 June 2017 7,297 5 View