MYH9(NM-IIA) is known for regulating cell migration. It can express both on cytoplasmic and membrane. If I can down-regulate MYH9 expression on cell membrane, will it effect its function?
In fact, I have found a secreted protein to co-localize with MYH9 on the membrane(Detected by confocal) and reduce MYH9 expression on the membrane(Detected by WB), and it has some oncogenic functions and effects cancer related pathway.