For all those intersted in cell research.... | Contact experts in Cell Biology to get answers
1,788 views 1,750 posts
Questions related to Cell Biology
I am doing proliferation assay, after i fix the cells with 4% paraform aldehyde, i stain the cells with Crystal Violet for 2 hrs, after washing excessive dye i air dry my plates and then use 10%...
07 August 2017 5,827 4 View
Hi there, I am doing a qPCR experiment and I am going to compare cytokine stimulated cells vs. unstimulated cells' gene expression levels. I need around 10 million cells (1 X 10^7) for RNA...
04 August 2017 7,440 2 View
My protocol didn't work when using SD-trp culture. And nocodazole may not work. I detect the phase of yeast cells by flow cytometry. I'm looking for a new protocol for synchronization of the...
27 July 2017 965 3 View
I'm using HepG2 to form 3D HepG2 aggregates with alginate, to confocal microscopy analyzes. However, I have difficulty to maintaining the spheroids after fixation and all procedures of incubations...
25 July 2017 570 1 View
Hi, I am developing a new assay, where I have to make some customized inserts to my culture plates. I want to make sure that these inserts will sink to the bottom of the wells, hence, I would like...
25 July 2017 6,559 4 View
I run a co-culture experiment with HeLa (mammalian) cells and bacteria (P. bivia, P. disiens, L. crispatus). Following a co-incubation period i want to measure the viability of the HeLa cells...
24 July 2017 6,542 3 View
One of the key factors in karyotype technique is to ensure that we have a high percentage of dividing cells to increase our yield of metaphase spreads.The use of colcemid, which prevents formation...
21 July 2017 1,097 7 View
I am trying to carry out trypan blue assay. However, when I did the experiment, I was so confused about what is dead cell. My protocol : I cultured MDA MB 231 cell lines in plastic dishes. After...
20 July 2017 8,382 6 View
I'm planning a glucose uptake assay with adult mesenchymal stem cells and possibly rat neural stem cells for my undergraduate thesis. i'd appreciate some recommendations for uptake kits and...
19 July 2017 2,338 1 View
Many publications describe the use of MCD to mimic steatosis in HEPG2 and other liver systems. There in the M&M people describe they order it from Gibco or Life tech. However, i can find it in...
12 July 2017 1,346 2 View
I need to isolate diatoms from the various other debris, wat are the possible methods? by using inverted microscope, How can we do ?
12 July 2017 6,631 3 View
I read that you culture cells on a non-adherent surface to promote pseudoislet formation, but won't the pseudoislets break apart after you spin them down and resuspend the cells? What type of...
11 July 2017 8,732 4 View
dormant cells don't undergo any metabolic processes, but an appropriate stimuli can activate the Partially dead cells, to fully functional cells. Regenerating/reviving a dead cell, using...
11 July 2017 8,898 1 View
I'm just trying to organize balanced panels to describe lymphocytes from my patients and I was wondering if its possible to detect autophagy with extracellular staining. I suppose Beclin 1, LC3 or...
10 July 2017 1,235 3 View
Im going to co culture CD4 naive cells with mesenchymal stem cells and will add T-helper 17 differentiation media. After 10 days, I will use the cells for further analysis. Do you think the...
07 July 2017 9,638 6 View
07 July 2017 191 3 View
07 July 2017 2,025 5 View
07 July 2017 2,722 2 View
07 July 2017 483 1 View
I have an issue. I need to measure cell viability of my HeLa (mammalian) cells that are in co-culture with bacterial cells (Prevotella, Lactobacillus). If used common MTT assay methods with a 96...
07 July 2017 8,075 4 View
I'm looking for an alternative for bacteria-bacteria interaction. I'm using transwell to study interaction between 2 bacteria, but I have a lot of contamination problem. I've been looking for...
07 July 2017 7,517 3 View
In most cases the cells are live but the medium get turbid, which leads to cell death, as i observed. The medium I use is DMEM-F12 with 15% FBS and 1% Strep-Pen as supplement.
07 July 2017 1,310 4 View
I tried different enzymes (Papain, Liberase, Accutase) but the protocols I used do not give me a good yield or they reduce a lot the viability of the neurons. Thank you in advance!
07 July 2017 4,748 0 View
yeast cell growth
07 July 2017 5,869 2 View