For all those intersted in cell research.... | Contact experts in Cell Biology to get answers
1,794 views 1,750 posts
Questions related to Cell Biology
asking on behalf of a colleague. what is the minimum no of cell required for extraction of RNA destined for RT-PCR
17 May 2017 7,596 2 View
presently we have trypan blue but i think its works mainly for animal cells as we did not find any color differentiation between dead and alive algae. we are yet to try Neutral red, i would...
16 May 2017 2,654 3 View
Hi, I have a simple question to which I seem having problems finding an answer online. Does MG-132 inhibit β5i (inducible) subunit of proteasome? thank you for any help!
14 May 2017 2,002 3 View
I am trying to stain Neisseria gonorrhea but not stain HEC1B or uterus cells. Is there such a dye that can do that?
10 May 2017 8,371 3 View
Could anybody recommend a good GFP antibody for zebrafish embryos immono-EM?
09 May 2017 1,883 1 View
Guys. What should I expect when I run LDH assay for nocodazole treated fibroblasts? An increased cytotoxicity or profile similar to cells+medium.. Bcoz I know that nocodazole induces mitotic...
07 May 2017 8,998 3 View
Can anyone suggest a method enabling a deprivation of leukocytes from the mouse bone marrow-derived cultures- my cultures contain both populations- some leukocytes and mesenchymal stromal cells, I...
05 May 2017 7,419 4 View
05 May 2017 2,429 2 View
05 May 2017 8,718 4 View
I'm trying to stimate the cell cycle profile of my cells and I wondered if the high melanin content of them can alter the fluorescence of propidium iodide. If so, does anyone know if it can be...
05 May 2017 7,506 2 View
Would anybody know a good AAV serotype and promoter that would preferentially infect progenitor neuronal cells in the dentate gyrus, DCX+ cells that could be imaged after 7-9 days after...
05 May 2017 7,989 0 View
05 May 2017 3,847 3 View
Is there any possible cell surface marker that can indicate that the cell expresses P16? I mean without permeability with ab and damaging the cell? I need the cells later for RNA - seq, so...
05 May 2017 7,286 2 View
Hello all, anyone who can advice me on how to get efficient transduction of AAV9-virus in skeletal muscle of adult mice? I saw paper talking about tail-vein injection but in my hands it did not...
05 May 2017 7,962 4 View
05 May 2017 7,126 1 View
Why two mountains occur in dcfda assay through facs ? my protocol is following 1. detached cell using TE and drug treatment in HBSS media. 2. after appointed time (1,2 h), dcfda staining during 30...
05 May 2017 6,641 3 View
Studying cell migration of glioblastoma primary cell lines and we're looking for alternatives to FBS to promote adhesion without altering the migration phenotype. Does anyone know the particular...
05 May 2017 9,500 3 View
Hi, We are trying to find a buffer that we can use to collect and lyse primary macrophages to assay the levels of cAMP accumulation. We have been using a kit from arbor assays that allows us to...
05 May 2017 7,206 0 View
I am trying to express protein on COS-7 cell membrane with a GFP tag. Can any one help me out on how can I purify the membrane protein for western analysis and may be even for flow cytometric...
05 May 2017 1,856 2 View
Suspension culture method
05 May 2017 590 1 View
n/a
05 May 2017 8,402 4 View
Hello every body, I want to perform a fibroblast-like colony unit formation or CFU assay or clonogenic assay for mesenchymal stem cells. Personally I am working with equine adipose-derived stem...
05 May 2017 507 5 View
05 May 2017 1,712 4 View
Halo everybody. I am doing immunocytochemistry staining of pluripotent stem cell-derived neurons seeded on coverslips. During ICC procedure, I fixed the cells using 4% PFA for 15 minutes and then...
05 May 2017 8,332 4 View