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Questions related to Cell Biology
I am trying to make 25ml aliquots of sterile spring water for some field work. I do not have access to a sterile hood, so I have been autoclaving spring water in 50ml PP/PPCO centrifuge tubes with...
20 July 2019 676 2 View
I am trying to grow an immortalized cell line that has done well before, but now are no longer attaching. The cells attach fine post thaw, but over the course of 48-72 hours, all the cells...
07 July 2019 9,807 7 View
Hi, Is there a good database for immortalized human cell lines? In particular cancer cell lines? Many times I need to look up attributes of a cell line; size, protein expression, doubling time,...
05 June 2019 1,133 5 View
Good evening, I wanted to ask you about something I have been observing. I work in a laboratory that does cell biology and molecular biology and we usually have some reagents and kits in the...
12 April 2019 1,355 3 View
Hello everyone! In most papers I looked in, it is mentioned that one of the main reasons why cells keep low resting cytosolic Ca2+ levels is that if high, it will interfere with ATP,...
04 April 2019 4,427 2 View
thank you so much!
03 March 2019 9,426 2 View
Cancer cells uptake of NPs time period?
25 February 2019 6,830 5 View
i was wondering that, if zygote, that divide through mitosis and generate somatic and germ cells, and in plants, somatic cells undergo meiosis to produce spores. then, is it possible that animal...
02 February 2019 9,757 5 View
I am trying to understand why if cells are exposed to a carcinogen equally, in the same duration, frequency and intensity, only some cell types show mutation or become cancer faster than others.
12 December 2018 9,902 7 View
Bonjour, SVP si quelqu'un a déjà travailler sur l'apprentissage de la biologie cellulaire chez les étudiants universitaires. j'ai besoin d'un modèle de questionnaire dans ce sens. Merci d'avance
12 December 2018 3,982 0 View
I am doing some Bioinformatical analysis with all the aquaporins of human. But I am getting confused with this two. I don't find any reliable source regarding this.
11 November 2018 3,987 3 View
We are working on a new compound for Alzheimer disease and use THP-1 cell line and Amyloid beta 1-42 from US peptide as stimulator. Since, US peptide does not provide this Amyloid beta anymore we...
09 November 2018 7,514 3 View
Hi, I am hoping to get opinions/input since I am undergrad and I see certain trends that I am highly curious about. I have been doing lab rotation to get an extensive research experience....
10 October 2018 9,860 0 View
In case of humans does any transposon type move genes that code for such human proteins that have functions? I've read at several sources that they transcribe the proteins needed for the...
15 September 2018 2,823 4 View
Im functionalising gold nanoparticles with thiolated DNA, this is done using excess DNA in solution, it is critical that once the procedure is complete any remaining DNA is completely removed....
09 September 2018 8,022 5 View
09 September 2018 3,517 4 View
Why can't both the translation and transcription processes occur A. Simultaneously in eukaryotes B. If not simultaneously, but at least in the same location inside the cell?
07 July 2018 1,203 2 View
I am trying to use CRISPR-cas9 in yeast a cell biology lab I will be teaching this fall . A few people I talked to said they didn't have much success and suspected that the problem was not...
06 June 2018 8,701 1 View
I am getting confused with these terms. Are they represent the same part of the ER? It is written "The cortical endoplasmic reticulum (ER) is an intricate network of tubules and cisternae...
05 May 2018 8,802 3 View
I want to enroll in a course to improve my knowledge in these fields and the possibilities for research and publication. I am also interested in statistics in medicine, using different programs...
04 April 2018 8,166 0 View
Heyho, I am new to the field of cell biology and am currently working on a script in ImageJ to analyse confocal images (of labelled cells). These are the parameters I am interested in: - mean...
04 April 2018 5,786 8 View
Methyl halide pesticide cell biology/molecular biology in insect
04 April 2018 1,114 5 View
I applied some mechanical stresses on cells and discovered that FAK and p38 MAPK are activated by phosphorylation. For the purpose of publishing, i feel that it will be great if i can look for...
04 April 2018 5,890 0 View
I am performing AFM force spectroscopy on red blood cells by immobilizing them on glass surface. I clean the glass coverslips by sonication in soap solution, Milli Q, ethanol, acetone, ethanol for...
26 March 2018 767 3 View