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Questions related to Cell Biology
I know annexin-v is used to identify its presence, but is there a way to actually quantify it and differentiate it from PS inside the cellular membrane? (Also, is there a membrane-impermeable dye...
07 July 2017 10,129 1 View
Hi there, I was wondering if the cells have the "spread-out" spider web looking morphology, does this mean that the cells are senescencing? Do they also have "bubbles" in their cytoplasm? Thank you,
07 July 2017 4,268 3 View
I always understood fold change calculations following a qPCR to be calculated by 2^-(ddCT). Is this incorrect, my mentor thinks it is? Potentially they are thinking of something else. Could you...
07 July 2017 7,978 6 View
I want to look at dendritic cells by flow cytometry. I've been using Collagenase IV plus DNase I in blank RPMI1640 media.
07 July 2017 7,313 2 View
I'm just trying to organize balanced panels to describe lymphocytes from my patients and I was wondering if its possible to detect autophagy with extracellular staining. I suppose Beclin 1, LC3 or...
07 July 2017 6,988 4 View
I need OP9 Cell line for differentiation of human iPSc to Hematologic stem cell
07 July 2017 5,435 3 View
I wonder is there any virus which will target specifically to hepatic cell?
07 July 2017 5,446 0 View
Hi All, how can I induce human dermal fibroblast to necroptosis? I read some papers and it is said that different methods can induce different cells to necroptosis. Thanks.
07 July 2017 4,643 2 View
I have been trying to block KDM6B and KDM5B with to chemical blockers (subretinal injection-rat). I could not detect any changes in treated eye vs control eye with Western Blotting for H3k27me3...
07 July 2017 7,281 2 View
Hello every one: I would like to know if the retroviral transduction on Mesenchymal Stem Cells is better option that lentiviral transduction. We need to inhibed the expression of some proteins of...
07 July 2017 9,364 3 View
Hi, I am developing a new assay, where I have to make some customized inserts to my culture plates. I want to make sure that these inserts will sink to the bottom of the wells, hence, I would like...
07 July 2017 8,320 4 View
Hello, I've been doing some exosome/vesicle extractions. I've done some before with PC3 cells, resulting in a tiny white pellet. However, this is the first time doing this with LNCaP. Following...
07 July 2017 3,894 3 View
Does anyone know of any intracellular solutions or methods for reducing or preventing LTP washout after going whole cell? Canoncial thinking suggests that after 5 minutes dyalisis of the cell...
07 July 2017 10,179 4 View
Suppose i seeded 5000 cells in RPMI medium, so how i decided that how much volume of RPMI medium for 5000 cells treated.
07 July 2017 1,372 2 View
I am trying to culture the granulosa cell from mouse. When I use the JC-1 to detect its' mitochondrial membrane potential, I can see two different populations, one has low MMP, while another has...
07 July 2017 5,889 1 View
I run a co-culture experiment with HeLa (mammalian) cells and bacteria (P. bivia, P. disiens, L. crispatus). Following a co-incubation period i want to measure the viability of the HeLa cells...
07 July 2017 8,910 3 View
Hello (I will say my genes of interest as A,B and C) Based on the results so far, A seems to induce translocation of B into nucleus from cytoplasm. As for B, B is already known as a...
07 July 2017 7,134 6 View
Dear all I've experienced several times that my human TERT-immortalized preadipocytes have a lower percentage of differentiation in 15cm plates, compared to 6-well plates. Have any of you...
07 July 2017 814 1 View
I am currently studying mutations in the RRAS gene and plan to functionally characterize these mutations; specifically, determine their effects on certain cancer hallmarks. To do this, I will be...
07 July 2017 2,030 2 View
I would like to get some practical info with regard to biolog PMM phenotyping. I also have done some and would like to share info too.
07 July 2017 1,345 2 View
I have performed a 10 day long treatment with MCF-7 cells in 24-well plates. I have senesced them, and followed by treatments with drugs that are expected to kill normal cells, but also have...
07 July 2017 3,175 5 View
One of the key factors in karyotype technique is to ensure that we have a high percentage of dividing cells to increase our yield of metaphase spreads.The use of colcemid, which prevents formation...
07 July 2017 6,667 8 View
I'm planning a glucose uptake assay with adult mesenchymal stem cells and possibly rat neural stem cells for my undergraduate thesis. i'd appreciate some recommendations for uptake kits and...
07 July 2017 1,549 1 View
Starting from single celled prokaryotic organims life in earth has undergone through a radical transformation and today we have eukaryotic cells.What is the future of eukaryotic cell in terms of...
07 July 2017 2,979 1 View