How to count cells in different slide stained with different antibodies and calculate percentage ?naked eye or software,which software? - FAQS.TIPS

Badges
Science topic

More Jia Wen-juan's questions See All

How to reduce the material coming out of the torque rheometer?

A physical mix was in vitro evaluated on a torque rheometer. The composition was predominantly recycled polypropylene (PP), yet the melt flow index (MFI) was found to be excessively high....

25 June 2024 10,154 0 View

Who is the use of Generative Artificial Intelligence in Qualitative Research?

One of the most intriguing frontiers in contemporary research is the application of Generative Artificial Intelligence (GenAI) in qualitative research. This technological advance offers tools that...

13 June 2024 781 2 View

¿Que enfoque metodológico me recomiendan para abordar los gremios tróficos en un área natural de BST?

Me interesa investigar la distribución espacial de los gremios tróficos de aves en un área natural específica de bosque seco tropical, analizando la abundancia relativa de cada gremio en las...

12 June 2024 9,313 1 View

Laser for perovskite module?

Hi, I'd like to know if someone knows about a company where I can buy an affordable laser-scriber for perovskite films and another thin film for the fabrication of modules. Many thanks,

31 May 2024 2,841 0 View

Fluorescence spectroscopy: signal from water?

In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...

16 May 2024 6,266 5 View

Can I calculate the high and low spin of the material in Quantum espresso?

I would like to simulate the high and slow spin configuration of a metal oxide. I am using QE for it, but in my research, I found that this kind of calculus is not possible. Or if it is possible,...

15 May 2024 6,497 3 View

Does anyone have a protocol for MPs assessment in fresh snow?

Dear colleagues Could someone send to me an already validated protocol for microplastic analysis in fresh snow that includes sampling and analysis in the lab? I suppose there is no need to digest...

15 May 2024 2,378 1 View

How can technology help hospitalized children?

How do you think technology can help hospitalized children?

10 May 2024 6,508 2 View

What mode can confocal microscopy use to observe metal surfaces?

How can confocal microscopy observe cells stained on metal surfaces

28 April 2024 2,804 1 View

How can confocal microscopy observe cells stained on metal surfaces?

How to observe cells stained on metal surfaces using confocal microscopy,How to adjust the confocal mode

28 April 2024 3,103 0 View

Similar questions and discussions

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View