Looking for different methods to reduce the metabolic activity of cells. Primarily HepG2 cells, but muscle or adipose tissue cells either.
Temperature would seem to be the most obvious way. Q10 temperature coefficients for most enzymatic reactions are in the vicinity of 2 meaning that for a 10 oC decrease in temperature, the cells' metabolic rate would decrease by half.
Thank you very much Gordon!
you could try lowering the serum concentration in the media, as it contains cholesterol
Thanks very much Delilah! I will try this!
Hi everyone, As part of my PhD project, I am conducting mixed ANOVA's in SPSS. I understand how to run the procedure and have been using the SPSS Survival Manual by Julie Pallant as my main...
10 November 2018 815 8 View
I understand that if you collect data pre and post-intervention from the same individuals, a paired sample t-test is the best option to compare differences between the scores. However, if a few...
06 July 2018 8,072 5 View
I have conducted a human intervention study and measured various physical and metabolic characteristics pre- and post-intervention. I would like to conduct stepwise backward regression in SPSS to...
31 December 2017 4,493 9 View
I have ran multiple mixed ANOVA's on results I obtained during a recent study. I understand how to conduct a mixed ANOVA but I'm having trouble with satisfying certain assumptions of this...
10 November 2017 4,222 4 View
Looking for recommendations of highly regarded academic/research conferences on physical inactivity, sedentary behaviour and health taking place next year in 2018.
10 November 2017 4,081 4 View
I have two intervention groups with uneven subjects. When I run a two-way RM ANOVA it takes the means of the smaller group thus I presume the ANOVA results are incorrect. Is there a way of...
03 April 2017 6,098 10 View
I need to establish some alternative or additional techniques for analysing biomarkers of insulin resistance in blood plasma.
04 May 2016 300 1 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View