Looking for different methods to reduce the metabolic activity of cells. Primarily HepG2 cells, but muscle or adipose tissue cells either.
Temperature would seem to be the most obvious way. Q10 temperature coefficients for most enzymatic reactions are in the vicinity of 2 meaning that for a 10 oC decrease in temperature, the cells' metabolic rate would decrease by half.
Thank you very much Gordon!
you could try lowering the serum concentration in the media, as it contains cholesterol
Thanks very much Delilah! I will try this!
Hi everyone, As part of my PhD project, I am conducting mixed ANOVA's in SPSS. I understand how to run the procedure and have been using the SPSS Survival Manual by Julie Pallant as my main...
10 November 2018 909 8 View
I understand that if you collect data pre and post-intervention from the same individuals, a paired sample t-test is the best option to compare differences between the scores. However, if a few...
06 July 2018 8,123 5 View
I have conducted a human intervention study and measured various physical and metabolic characteristics pre- and post-intervention. I would like to conduct stepwise backward regression in SPSS to...
31 December 2017 4,553 9 View
I have ran multiple mixed ANOVA's on results I obtained during a recent study. I understand how to conduct a mixed ANOVA but I'm having trouble with satisfying certain assumptions of this...
10 November 2017 4,283 4 View
Looking for recommendations of highly regarded academic/research conferences on physical inactivity, sedentary behaviour and health taking place next year in 2018.
10 November 2017 4,143 4 View
I have two intervention groups with uneven subjects. When I run a two-way RM ANOVA it takes the means of the smaller group thus I presume the ANOVA results are incorrect. Is there a way of...
03 April 2017 6,152 10 View
I need to establish some alternative or additional techniques for analysing biomarkers of insulin resistance in blood plasma.
04 May 2016 372 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View