Cancer

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Questions related to Cancer

Is there any Cas9-based editing protocol for mouse primary neutrophils?

Recently I find one very interesting target that I want to verify in mouse neutrophils through Cas9-knockout, however, I couldn't find any practical in-vitro gene editing method for mouse primary...

25 July 2024 6,575 0 View

If we use entangled photons for the study of complex media (such as biological samples) does it helpful?

When light passes through complex media, surely there will be scattering phenomena taking place. If we use entangled photon pairs for such a study will it be effective? Also to enhance the flow of...

18 July 2024 988 0 View

Algebraic solution of Maxwell's equations?

At least some differential equations have the equivalent algebraic expressions. The sets of such (partial) equations are not excluded.

20 June 2024 4,332 4 View

Hi All, about multiplex immunofluorescence,what could be the reasons for unwated flurophore background ? other than autofluorescence and 1ry Ab conc?

Could the tissue quality/age be an issue ??

16 June 2024 7,820 0 View

How to adjust or recalibrate negative MFI values from cytometer?

I am looking at puromycin incorporation rate by flow cytometry using a specific anti-puro-antibody which I use to stain different cell types. For some cells the signal is high (i.e. Puro MFI),...

13 June 2024 1,185 1 View

The problem at hand concerns the application of the "II Lagrange's formula". Would you like to try solving it?

In triangle ABC, the bisector AL₁ is drawn. Points O₁, O₂, O are the centers of the circles circumscribed around triangles ACL₁, ABL₁, ABC, respectively. The radii are denoted as R₁, R₂, R for...

13 June 2024 7,778 1 View

What are the answers to Epidemiology Question including revalence, case fatality etc ?

Suppose that in January 1996, 1000 adult residents of a community accepted an invitation to be examined for hypothyroidism at a local clinic. Eight persons were found to have the disease, it was...

28 May 2024 7,021 7 View

What happens to general relativity time operates differently in gravitational fields & difference between general relativity & special relativity?

What happens according to general relativity time operates differently in gravitational fields and difference between general relativity and special relativity?

02 May 2024 812 2 View

Lesson study approach as a teacher development model for grade 6 mathematics teachers: A case of suing long division method?

Lesson study teacher development approach

14 February 2024 6,988 0 View

Could you please provide details on the optimal Lipofectamine 3000 transfection protocol for efficiently delivering a plasmid into CaSki cells?

Dear colleagues, I need to transfect a 10 kb plasmid into Caski cells and am hoping to use Lipofectamine 3000 for the transfection. If anyone has experience using Lipofectamine 3000 specifically...

12 January 2024 1,802 1 View

Journal article request please ?

Tous-Espelosin, M. et al. (2023) ‘“It Helped Me to Disconnect My Mind from the Problems”: The Subjective Experiences of People with Schizophrenia Taking Part in a Concurrent Exercise Program’,...

18 December 2023 894 2 View

Conjunction of autocorrelated residuals and factor analysis/SEM in psychometrics?

Basically most studies of "structural equation modelling + time-series data" focus on longitudinal datasets (e.g., (residual) dynamic SEM). However, for a questionnaire survey or measure at a...

22 November 2023 8,042 0 View

Related studies and related literature on learners agricultural competencies and challenges in agri-crop production 2019 up to present?

related studies and literature

11 November 2023 2,111 1 View

What practical hurdles for Lentivirus-Tiam1 therapy?

Lentivirus-Mediated Gene Delivery is a common method for introducing therapeutic genes into cancer cells. One such gene is Tiam1, known for its potential impact on cancer cell behavior.

17 August 2023 2,436 1 View

Why Salmonella typhimurium TA102 in my lab is NOT histidine dependent?

Dear experts, We bought Salmonella typhimurium TA102 from ATCC for Ames test. Upon checking its genetic analysis, turned out it is histidine independent which I was expecting histidine...

14 August 2023 9,754 4 View

Which human cell lines are best for invitro toxicity screening for drug in local musculoskeletal (mainly bone & muscle) application?

I need to screen the toxicity of several compounds that will be applied locally to an open wound of bone and muscle injury, which human cell lines (good for several passages) would be most...

27 July 2023 4,853 4 View

Should we divide the data among exploratory factor analysis and exploratory factor analysis or is the same data is used for both?

I'm doing a validation study and for construct validity, I'll analyze my data through EFA and CFA. So, the same data should be used in both processes or should we divide the data? And if we divide...

08 June 2023 7,090 5 View

What kind of cell culture contamination could this be?

Dear all, since a few weeks, we have been noticing the following 'stuff' swimming in our flasks (see images attached). The cells we use are self-isolated primary human keratinocytes. They are...

11 May 2023 9,142 0 View

Do microorganisms use to remove pollutants that contaminate water soil&air&process of converting environmentalpollutants toharmlessproductsbymicrobes?

Do microorganisms use to remove pollutants that contaminate our water soil & air & process of converting environmental pollutants to harmless products by microbes?

03 April 2023 4,719 4 View

Protonation states of histidine?

how to determine protonation states of histidine

10 March 2023 7,396 0 View

Why is GATK4.3 give inconsistent outputs?

When I run ApplyBQSR two of my samples generate all the chromosome lists and then MT. For the other samples that came from a similar pipeline, MT is not available in the list. Surprisingly, the...

23 February 2023 6,783 0 View

Methods for calculating adjusted incidence rates using validation statistics?

I am looking for advice on methods for adjusting incidence rates based on data obtained from validation studies (i.e., sensitivity, specificity values). Any insight you can provide would be helpful!

06 December 2022 9,152 4 View

I have a multigene alignment of approximately 30k bp length. Can I run Phylobayes with this dataset in my PC?

I have no idea how to run Phylobayes. Any pointer will be really helpful. I am not getting any quick start guide for this software nor the manuals are very clear to me. I am really wondering where...

21 April 2022 3,773 0 View

Outliers in independent variable (Logistic regression analysis)?

Hello, I would like to conduct a logistic regression, however, I have an independent variable that is not normally distributed and some values seem to be a bit extreme. Shall I be worried about...

23 March 2022 8,443 4 View

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