I have extensive experience in WISH and FISH in zebrafish embryos when it comes to mapping the expression of genes within the fish. I am now about to start a new project, checking the expression of genes within zebrafish skin cells. For that, I need to be super careful with the permeabilization. Does anyone have any optimised protocols for whole mount hybridization on SKIN?
Many thanks
Hi Monika,
How old are your samples (larvae, juveniles adults?). I would look at protocols from labs that study pigmentation. In particular David Parichy's lab has well documented protocols on their website with helpful guidance to different stages of juveniles (http://dparichy.as.virginia.edu/lab-protocols.html). If you want to look at adults, you may have to perform sections. In our hands paraffin sections have worked really well for colorimetric or Fluorescent ISH. I'd be happy to provide protocols for those if you need them.
Good luck
Sandeep
- Badges
- Science topic