Actually I work in bacteriophage resistance mechanism. So I found my bacteria look like different structure show in normal nutrient ( 1x LB) and lower nutrient (0.25x LB). When i used bacteria culture for TEM from 0.25x LB broth, it showed look like type IV pilus present. As I did not find any motility effect for my bacteria sample, but shows like conjugation tube or type IV pilus. But how can I prove that is the structure of conjugation tube or type IV pilus ? My bacteria is Acinetobacter baumannii.