Serial peritoneal lavage on the same animal should not be performed. Ethics committees will not permit it. Peritoneal lavage should only be performed post euthanasia.
Im not sure what you need the cells for, but I have only heard of peritoneal lavage on dead mice. I would think that it could be done on a live mouse (it is no more invasive than abdominal surgery) but it depends on your ethics committee.
If you are after a serial source of infiltrating monocytes/macrophages, measuring chemoattractants or infection, you could consider the 'air pouch' model where a subcutaneous space on the back is inflated with air and exudate can be washed and collected serially.
Hi Natalie! Thanks for your reply! I was wondering if you know the optimal volume (mL) for peritoneal lavage on dead mice (7-8 weeks of age).
Thanks!
I've usually done no more than 3mL in a mouse of that size, usually 2 (my research is more interested in examining what cells are in there than collecting every last one). To get a good yield you could do 2-3 washes of 2mL (however for a live mouse I would think one wash would be the max).
Hey I found this paper where animals were lavages under surgical conditions and then kept for a survival study. Article Peritoneal Wash Contents Utilized To Predict Mortality in a ...
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