Is there any research about Psychological Capital as predictor of Career Adaptability among college student?

I'm looking for some research that has the same question, about psychological capital as a predictor of Career Adaptability among college students.

10 September 2020 2,684 3 View

Would anyone recommend me a comprehensive book on Machine Learning using big data and R?

I am an epidemiologist and now diving in the ML technique. I am used to working with R, and even though I have a feeling that most content on ML is broadly available using Python, I would like to...

25 June 2020 4,853 10 View

How would you ensure that Gibson cloning of multiple fragments is done in frame?

Hello! I would like to clone a very large fragment of DNA into my vector (which will be digested with restriction enzymes) . I will need to amplify this fragment by PCR in pieces (2 or 3) and then...

26 March 2020 4,832 2 View

Why a docking complex is unstable in MD?

I have a complex with good energy binding, but this one breaks when I run a MD in namd, the RMSD is about 10 or more. The configure file for namd was maked with gwikMD.

12 June 2018 657 7 View

Hello. My concern is whether the long LacO arrays can really form a proper chromatin structure with nucleosomes in budding yeast. Thanks. Fabiola

15 May 2017 3,008 2 View

How expensive is it to work with Zebra finches?

I'm trying to evaluate the cost-benefit of working with this model in the lab.

26 September 2016 6,211 3 View

which is the most reliable oligonucleotide design program?

i need to desing the primers for the production of a recombinant protein, any advice??

13 April 2016 762 3 View

Where can I get the xylene cyanol?

11 August 2014 6,384 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View