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Questions related to Cancer
I tried several times to infect my mesenchymal stem cells with EV-71 virus. But every time I did not find any Cytopathic effect on my cells. Here, I used MOI-1 and MOI-10. What can be probable causes?
25 February 2022 2,323 1 View
I have recently done some inhibition experiments with MRTX849 and AMG510 in MiaPaCa-2, H358, HCC44 while looking at the phosphorylation of ERK1/2 and S6. Once over 24h and once over 96h with...
23 January 2022 562 0 View
In stark contrast to other land plants Selaginella and most liverworts are devoid of whole-genome duplication. What is the explanation and what is role of polyploidy in survival of ferns
05 January 2022 2,676 4 View
I'm having trouble getting clear images of my bacteriophage under TEM. The process I've followed thus far is described below. Any help would be appreciated. I've isolated bacteriophage active...
13 December 2021 8,470 3 View
I am analyzing survival outcomes using GraphPad Prism software. The software automatically generates the Kaplan-Meier survival curve along with the associated hazard ratio and p-value. I am...
09 December 2021 7,257 3 View
We have confocal z-stacked images of mesenchymal stem cells from where we need to find the z value or the depth of the cell. We need the height values from the z-stacked cell images. How can we...
01 November 2021 4,043 4 View
I have genetic distance for several species which needs to be integrated with the morphological data that I have measured. I need to see whether there is a correlation/ trend between the Genetic...
17 September 2021 9,741 3 View
Hallo! Does someone have experience culturing cells derived from infectious samples? I would like to isolate the bone marrow mesenchymal stem cells from patients diagnosed with bone infection and...
26 August 2021 9,373 9 View
Hello everyone. I am planning a one-group pre-/post test design (I am aware of the limitations, including a control group is unfortuntately not feasible) and want to measure whether var 1...
21 August 2021 1,944 4 View
I have performed MTT assay on Ti samples and now I want to reuse those samples, can anyone guide me how I clean the samples and re-use it. Thanks
07 August 2021 2,142 5 View
Hello everybody, Right now, my team and I are working on a research project that established a primary culture of mesenchymal stem cells from murine adipose tissue. We executed the establishment...
01 June 2021 2,944 2 View
In an in-vivo study against MCF-7 Breast cancer, it was observed that the response to the targeted drug containing surface engineered silver nanoparticles was highly different in three mice as...
17 May 2021 4,055 10 View
I conducted a survey using a questionnaire. My target population is online mobile gamers in a certain area. The population is unknown, so I do the sampling. My research is analyzed using logistic...
05 May 2021 3,763 3 View
I am trying to use bone marrow aspirates to make a "tissue microarray (TMA)". My hope is to stain genes by IF on multiple samples at one time -so not a smear. I have used TMA for solid tumors...
15 February 2021 4,916 0 View
I am running my plates with 4 technical replicates. How can I tests these 4 for outliers and still be MIQE compliant. I can't seem to find any published work that shows how outliers were determined.
09 February 2021 6,459 2 View
Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...
29 January 2021 9,063 4 View
Hi all, I'm trying to perform an IHC on inflammatory breast cancer tissue microarray (TMA). Do you know any company/ies that normally has/have this specific TMA in stock, or could construct. The...
11 December 2020 8,202 5 View
Hi, I am looking at a SNP located within the 3'UTR of a gene of interest. I discovered that in a tissue type of interet one of the alleles of the SNP is preferentially expressed over the other in...
23 November 2020 9,740 0 View
Previously "TCGA2STAT" a tool of R is used to download TCGA cancer data and their corresponding normal samples, but this tool is deprecated now. Now I need to download a lot of cancer samples as...
12 November 2020 1,052 5 View
When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...
28 October 2020 2,359 2 View
If Mesenchymal Stromal Cells (MSCs) are used in cell therapy, what are the stromal cells and in what proportion they are recommended for cell therapy? I appreciate the guidance, reference,...
15 October 2020 627 2 View
Dear, researchers i want to introduce ovarian cancer in Swiss mice, because most of carcinogens are not organ specific, can anyone tell me which drug/carcinogens i try to introduce cancer?
13 October 2020 5,151 3 View
I am trying to evaluate the performance of a screening tool for meningitis. For this purpose, I have separate data on bacterial, viral and TB meningitis. I am interested in calculating the...
08 October 2020 2,008 1 View
Can radiotherapy be used in the management of Vulval Intraepithelial Neoplasia (VIN), especially in patients with extensive disease or those who are not candidates for surgery or repeat surgery
28 September 2020 8,474 5 View