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Questions related to Cancer
I am checking Salmonella typhimurium TA98 strains for the Ames test. On GM plates (excess histidine, no biotin) (H plate). I did not observe any colonies (as expected), but I noticed irregular...
last month 6,623 0 View
How should concept papers and policy papers be developed to align with the priorities of state agriculture, ensuring evidence-based recommendations for policymakers?
last month 3,657 1 View
I'm currently working with mesenchymal stem cells in culture and want to confirm their identity using specific markers. Could anyone suggest the most commonly used surface markers or protocols...
30 July 2025 8,607 2 View
What to do if single stain control using endogenous reporter do not show good positive peak
19 July 2025 7,955 3 View
Native tracheal cartilage shows strong bluish-purple color in H&E staining. but decellularized tracheal cartilage tissue post transplantation does not show bluish-purple color. The...
20 May 2025 4,171 1 View
I am seeding 2000 OSCC cells in a 96 well plate and adding drugs at varying concentrations along with a negative control (only media). I am incubating the cells woith drugs for 96hrs and then...
28 April 2025 9,494 1 View
Hi everyone, I am trying to do an MTT with caco-2 cells. I am following the same general protocol for an MTT for a 24 well plate. 1. Seed cells 48h 2. Add 100ul of MTT solution 3. Incubate...
25 April 2025 6,855 2 View
Pathology and Laboratory Medicine (PLM) e-IЅЅΝ: 2640-4478Gold ОА Peer-rеvіеwedDear Mollica, Corrado,Happy New Year to you!Researchers can publіѕh their work for a global readership across a...
30 March 2025 8,646 8 View
I am using CD45,CD31,Sca1,PDGFRa (negative selection) and a7Inegrin+ as panel to quantify satellite cells. But when I validate this with Pax7CreERT2;tdTomato mice(which labels satellite cells with...
04 February 2025 7,602 3 View
Hello, my iPSCs always seem to migrate to the center of my well despite shaking my 6-well plate (in an infinity sign shape) quite meticulously before I stick them in the incubator. I always check...
27 January 2025 6,482 3 View
Dear all, I am currently investigating LPS-induced cell death. To determine the IC50 value, I seeded a cancer cell line and treated it with various concentrations of LPS as outlined in the...
07 January 2025 9,015 4 View
NeuroBridge is a brain-computer interface designed to translate thoughts by sensing brain activity via a headset and converting it into text or speech displayed on a smartphone. It aims to help...
07 December 2024 4,097 1 View
Suppose I want to perform MTT of suspension cells. In which I seed cells in colourless media supplemented with 10 % FBS. Now I cant discard media while adding MTT. In that case FBS is present...
24 November 2024 3,502 2 View
I am a student studying at Shu-Te University and is currently conducting a study regarding beauty filters. I'm looking for validated questionnaires to serve as bases for a survey.
23 November 2024 4,504 1 View
Sana po makatulong
20 November 2024 7,455 1 View
The question is if the Relativistic Kinetic Energy formula: KE_rel = (gamma -1) mc**2 Has it been verified using thermal calorimetry? SINCE I WROTE THIS QUESTION, I FOUND OUT ABOUT THE BERTOZZI...
18 November 2024 5,905 5 View
I am facing challenges in isolating neutrophils from human peripheral blood after addition of Histopaque 1077. Neutrophils at the bottom of the tube after centrifugation are mixed with red blood...
21 October 2024 6,038 3 View
Hello. I've stained these cells with a 1:1 volumetric ratio of trypan blue to culture media shortly after resuspending during passaging. They appear alive to me at 4x magnification (cells are...
15 October 2024 4,121 0 View
I am currently working on the identification and characterization of Spirulina (Arthrospira platensis) species and would like to identify reliable primers for this purpose. I am particularly...
10 October 2024 2,178 0 View
I thawed my SH-SY5Y cells in DMEM with 50%FBS in T25. The cell morphology after thawed for 24 hours look good and I changed medium with DMEM with 50%FBS. I maintain the cell until it reaches...
24 September 2024 9,496 0 View
My name is Sharifah , Malaysian medical graduate from Turkey. I need a sample of published survey questionnaire in the topic mentioned for my research. Any help is highly appreciated. Thank you.
07 September 2024 7,780 0 View
I use sanger method regularly to sequence DNA but this time I came across a problem which I couldn’t find any sense I did sequence different segments of DNA double strands that belongs to...
02 September 2024 8,026 3 View
This study focuses on the instructional competencies of the master teachers in the Philippines
13 August 2024 603 2 View
13 August 2024 2,031 0 View