Cancer

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Questions related to Cancer

Why bone marrow cells arrest at DN3/DN4 stage of T cell differentiation when grown on OP9-DL1 cells?

I am using the OP9-DL1 co-culture system to differentiate mouse bone marrow cells into double positive T cells. I infected the bone marrow cells with a retrovirus, selected the GFP+ cells, and...

11 August 2017 6,738 1 View

How to generate Super Enhancer mediated lncRNA network to select functional modules as biomarkers?

How can we generate super-enhancer mediated lncRNA network to detect prognostic biomarkers in cancer via indication of upregulated / downregulated gene states....

10 August 2017 320 1 View

Qpcr statistical analysis

I have got ct values for Experimental gene in cancer samples, GAPDH in cancer samples, Experimental gene in control and GAPDH in control.Now I know about the double Delta Ct method. We will get...

10 August 2017 1,664 8 View

Can anyone recommend a good protocol for C.elegans spike in for isolated miRNA from tissues?

I have an isolated RNA, extracted from human tissues biopsy. I want to determine the expression of miRNA by qPCR by using miScript miRNA PCR Array by Qiagen, and C. elegans spike-in is used as one...

10 August 2017 8,584 1 View

How do you look for snps in a particular gene in hap map cell lines using 1000 genome browser?

I need to look for snps and copy number variants in a few genes in hap map cell lines. Can anyone guide me over using it?

04 August 2017 8,756 0 View

Is there any chance that somatic mutation will change to a normal gene without any mutation?

May be a general question; sometimes stupidity too.... For example, cancer is caused by somatic mutation in the genome. And treatment has been given to the patient. So what is the chance of change...

18 July 2017 7,861 4 View

Which tests do you believe to be useful in the investigation of recurrent miscarriage?

Facing recurrent miscarriage is not easy and patients are usually submitted to a vast number of tests aiming to find a cause. As a matter of fact, literature does not support so many tests in the...

18 July 2017 6,189 1 View

Silencing of Cas9 in mammalian cells?

I've heard from several independent people that their Cas9 cell lines that constitutively express Cas9 is being silenced. I've heard it for neuronal stem cells as well as for HEK293 cells. I also...

18 July 2017 4,035 2 View

How do you effectively inject cells into SCID mice via tail vein injection to develop a CML model?

I am trying to establish a cell line based xenograft model of CML in SCID mice. I tried to inject 5x10^5 K562 cells (in saline for injection) but there was considerable difficulty in injecting the...

14 July 2017 1,701 3 View

How we measure the organ dose from CTDIw on impact CT patient software

impact CT patient DOSIMETRY CALCULATOR  software

13 July 2017 2,246 4 View

How can I remove an article from the list?

How can I remove an article from the list?

11 July 2017 6,993 3 View

Is there any medicine or somall molecular can disrupt mouse immune system totally to mimic SCID/NOD?

For some reasons, I need to use C57 or DBA mice with impaired immune system for organ transplant from other species such as Rat or monkey. SCID/NOD mice, however, can not be used in my exprements....

10 July 2017 2,076 4 View

Does anyone know how can I eliminate tumor associated Fibroblasts in primary culture of human bladder tumor?.

I want to isolate and culture cancer epithelial cells from tumor.

04 July 2017 4,773 3 View

Is there any formular for calculating bleeding time of blood?

The bleeding time was tested in wistar albino rats using the Duke method. The time before bleeding and after bleeding was noted. To statistically get the differences, what formulars can be used?

03 July 2017 6,933 3 View

Why most omics studies end up with lists of differentially expressed "genes"?

I would like to know your answer to the following questions:  Why most omics (e.g., microarray, NGS, PCR) studies concerning disease diagnosis, or prognosis, or response to treatment end up with...

29 June 2017 3,011 6 View

Do chalcones have selectivity towards any particular enzyme?

I am interested in chalcones and their activities. I am amazed  with their therapeutic potential but really got a doubt whether they have  selectivity  towards any particular target?

28 June 2017 2,514 1 View

How biochemistry of Caffeine is associated with the cancers, as most of the studies shows the preventive role of it in cancer

How caffeine is associated with Cancers

19 June 2017 1,551 1 View

Nichotherapy for cancer. Is metformin suitable agent?

Metformin's anti-cancerous properties have been demonstrated in various cancer cells in vitro, such as lung, pancreatic, colon, ovarian, breast, prostate, renal cancer cells, melanoma, and even in...

17 June 2017 5,073 3 View

How to determine the concentration of an inhibitor?

I want to calculate the IC50 of a inhibitor compound. The inhibitor was obtained through insilico approaches. Now I need to perform the validation study for the inhibitor. To perform the assay I...

06 June 2017 1,243 1 View

Can someone help me with static anode x-ray tube spectra?

I need to perform Monte Carlo simulations of a imaging device (dental) with static anode x-ray tube.  Can you provide me one or point out a reference/paper/article? Thanks in advance!

05 June 2017 7,109 8 View

Do mouse embryonic fibroblasts express pluripotency markers?

Hello, I am doing flow cytometry to evaluate the pluripotency of mouse embryonic stem cells (mESCs) which have been derived and cultured on mouse embryonic fibroblasts (MEFs) Unfortunately, I am...

01 June 2017 2,518 4 View

Cell cycle dependent proportion of DNA repair via NHEJ and HR in plants or yeast?

Dear all, Does anyone know a publication/ data in plants or yeast on the proportions of DNA repair via NHEJ and HR in different stages of the cell cycle comparable to the findings of Mao et al....

31 May 2017 9,448 7 View

WHAT best method for induce cancer in prostate of Rat?and how long time for induction cacner in prostate of Rat

exercise and prostate cancer in animal model, and how long time for induction cacner in prostate of Rat

25 May 2017 1,524 3 View

Is there an RNA-seq database on cancers that includes patient's county/general location?

I am looking for something like NCI's TCGA databases (with patient county / area). I know any private / personal identifying information is removed but if anyone has an idea, please let me...

22 May 2017 1,455 0 View

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