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Questions related to Cancer Research
i m searching for papers that have an injection dose of stem cells that used to treat colon cancer ..
02 January 2017 1,355 3 View
I want to use it as cancer inducer
30 December 2016 5,102 6 View
we are working on cervical cancer ,we would see the expression levels of target genes in both control and patient samples. we are planning to use internal control as Coiled-coil-helix-coiled...
22 December 2016 4,432 7 View
Dear Scientific Community :) A colleague recently tested his melanoma (ME1402) cells for mycoplasma and obtained some strange images of rod-like structures - quite different from the 'dot-like'...
15 December 2016 4,739 3 View
Please refer me any protocol for developing colon cancer animal model in particular to mice and zebra fish where I can study metastasis.
14 December 2016 6,644 3 View
The Catalog # of it is 2524. It recognizes a protein which is highly expressed in wild-type mouse hepatocytes. The p53 level in wt hepatocytes is not detectable by western blot but this this...
14 December 2016 8,618 4 View
I am extracting RNA from skin cancer samples. The pipette was contaminated with crystal violet (the undergrad might have sucked in crystal violet while working with it). Now I am facing a problem...
14 December 2016 3,000 4 View
I am staining for Pax7 (fluorescence), and was looking for a negative control to establish the staining protocol. Any suggestion would be highly appreciated. Thanks a lot
11 December 2016 737 2 View
I've begun to establish some PDX lines that consistently passage through mice. At this point, I'd like to make a cell line from these PDX tissues. When I dissociate the tumor tissue and plate it I...
09 December 2016 5,413 3 View
How should I calculate the cut off for lymph node ratio in colon cancer, using a national data set?
09 December 2016 6,117 3 View
I have values (SI) of 4.18 and in the literature and found ranges from 0.02 to 18 in my area.
30 November 2016 7,554 3 View
i read many types of viruses causes carcinogenics i did not find the best and simple answer for integration virus tumor mechanisms
26 November 2016 2,745 3 View
In MTT test we can measure the toxicity of plant crude extracts, at which concentration we can consider the crude extract with potent, remarkable, good, weak or no activity? is there a scale...
26 November 2016 909 4 View
I heard that we have to passage until 50 passages in order to claim that the primary breast cancer cells become an immortalized cell line. Is this correct? Where can I find this information? I...
21 November 2016 2,507 5 View
I do western blotting with these lysates and i just want to minimize the degradation of protein as much as possible and i also want to know whether the storing temperature (-20 or -80) have any...
14 November 2016 3,903 12 View
I used antibodies to detect Ki67 in colorectal cancer and in normal tissue In the normal colon the Ki67 is found at the bottom of the crypt... and H3 is found in some mitotic cells. However, in...
12 November 2016 5,684 1 View
Does anyone have any experience with RET immunohistochemistry? I am interested in staining nerves in skin. Thank you
10 November 2016 7,862 2 View
I am currently trying to study tumor regression in mice xenograft models using treatment with hydroxyurea. There seem to be limited publications and I am trying hard to determine a suitable dose...
10 November 2016 2,036 3 View
Do u use special kind of glass slides, how do you level/flatten the molten agarose, how much volume of agarose do you use, if you dip your slides how do you achieve this (they drip off the slide...
10 November 2016 8,078 8 View
Dear all, I am trying to do western-blot for DNMT1 in cells treated with Decitabine and untreated cells and I am using a nitrocellulose membrane but I am getting a lot of non-specific staining....
10 November 2016 2,683 4 View
cancer reseearcher
07 November 2016 7,092 8 View
I'm injecting a hypomethylating agent for my project into transgenic mice. I'm struggling with handling the mice so that it can flip over on me and cause me to lose the injection site.
02 November 2016 4,359 3 View
What is the most important and most used cancer markers (proteins) in human blood plasma that is in use today, and which do you think is going to be the most important cancer markers in blood...
31 October 2016 355 8 View
Hello, I am trying to grow xenograft tumors with cells mixed with matrigel. I see that at the injection site, the gel sets and forms a bump. How do I measure the tumor growth accounting for the...
28 October 2016 724 5 View