I am extracting RNA from skin cancer samples. The pipette was contaminated with crystal violet (the undergrad might have sucked in crystal violet while working with it).
Now I am facing a problem which is a less intense 28S band on the agarose gel for my samples. Is that because of crystal violet? and do you think this crystal violet intercalation with RNA would affect the RT step and PCR?
Thanks
I hope you have more skin samples. Who knows what that pipette was contaminated with, and what's now contaminating your samples. At this point it doesn't matter much - you know they're contaminated and whatever you were doing with them is compromised. Sorry.
I hope you have more skin samples. Who knows what that pipette was contaminated with, and what's now contaminating your samples. At this point it doesn't matter much - you know they're contaminated and whatever you were doing with them is compromised. Sorry.
Hello Mayassa
Thi contamination will affect the performance of the PCR. Because, the cristal violet affects the composition of reaction buffer and this is importante in maitain the activity of polimerase enzime and others components. I hope you have more samples. Sorry
Best regards
Because your samples are contaminated with CV, I think your experiment must be performed using another samples. Using such contaminated samples will give you wrong results.
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